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4880

Quantitative Estimation and Validation of Rafoxanide in Presence of its Alkali-Induced Degradation Product by Different Spectrophotometric Methods

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Last updated: 03 Jan 2025

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Abstract

Objective: Development and validation of four simple, accurate, selective and sensitive UV spectrophotometric methodsfor the determination of rafoxanide in the presence of its alkali-induced degradation product without preliminary separation. Methods: (A) Dual wavelength method, where the difference in absorbance were calculated at 242 and 281 nm. (B) Area under the curve method,where area under the peak were measured at wavelength ranges (225-235 nm) and (275-295 nm). (C) Bivariate method, where the absorbance values were measured at selected wavelengths 250 and 280 nm. (D) First derivative method, whereThe amplitudes were measured at 253 nm. Results: Good linearity was found in the concentration range of 2.5–25 μg/mL for all the methods. The specificity of the methods were assessed by analysing synthetic mixtures containing the drug and its degradation product. Methods are validated as per ICH guidelines and accuracy, precision, repeatability and robustness are found to be within the acceptable limit. Conclusion: The proposed methods have been found to be accurate, precise and can be used for determination of the drug in pure form and pharmaceutical formulations as well as in the presence of its degradation product without any preliminary separation steps.

DOI

10.21608/aprh.2018.4880

Keywords

area under the curve, bivariate, Dual wavelength, First derivative, Rafoxanide

Authors

First Name

Mohammed

Last Name

Nassar

MiddleName

-

Affiliation

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University,11751, Nasr City, Cairo, Egypt

Email

mw-nassar@yahoo.com

City

-

Orcid

-

First Name

Khalid

Last Name

Attia

MiddleName

-

Affiliation

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University,11751, Nasr City, Cairo, Egypt

Email

dr.khalid64@yahoo.com

City

-

Orcid

-

First Name

Ahmad

Last Name

Mohamad

MiddleName

-

Affiliation

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University,11751, Nasr City, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

Ragab

Last Name

Said

MiddleName

-

Affiliation

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University,11751, Nasr City, Cairo, Egypt

Email

ragabmsaid@yahoo.com

City

-

Orcid

-

First Name

Ahmed

Last Name

Abdel-Monem

MiddleName

-

Affiliation

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University,11751, Nasr City, Cairo, Egypt

Email

ahmednaggar111@yahoo.com

City

-

Orcid

-

Volume

2

Article Issue

1

Related Issue

824

Issue Date

2018-01-01

Receive Date

2017-11-24

Publish Date

2018-01-01

Page Start

20

Page End

27

Print ISSN

2357-0547

Online ISSN

2357-0539

Article File

APRH_Volume 2_Issue 1_Pages 20-27.pdf

PDF . 623.4kB

Link

https://aprh.journals.ekb.eg/article_4880.html

Detail API

https://aprh.journals.ekb.eg/service?article_code=4880

Order

2

Type

Short Communication

Type Code

357

Publication Type

Journal

Publication Title

Journal of Advanced Pharmacy Research

Publication Link

https://aprh.journals.ekb.eg/

MainTitle

Quantitative Estimation and Validation of Rafoxanide in Presence of its Alkali-Induced Degradation Product by Different Spectrophotometric Methods

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Type

Article

Created At

22 Jan 2023