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4042

Validated Spectrofluorimetric Method for the Determination of Cefoxitin Sodium in Its Pure Form and Powder for Injection via Derivatization with 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl)

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Last updated: 22 Jan 2023

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Abstract

Objectives: An accurate and precise spectrofluorimetric methodwas developed and validated for the determination of β-lactam antibiotic named; cefoxitin sodiumin its pure form and powder for injection. Methods: Based on nucleophilic substitution reaction of target drug with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) to form a highly fluorescent fluorophore measured at 540 nm after excitation at 460 nm. Results: Under optimum condition, the proposed method obeys Beer's law in range (0.5-7 µg mL-1) and the reaction mechanisms were presented. Conclusion: The method was validated according to ICH guideline for accuracy, precision and was successfully applied for the determination of the drug in its pure form and powder for injection. The obtained results were statistically compared with those of the reported method and found to be in good agreement.

DOI

10.21608/aprh.2017.4042

Keywords

Cefoxitin sodium, Spectrofluorimetric method, 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole, NBD-Cl

Authors

First Name

Khalid

Last Name

Attia

MiddleName

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Affiliation

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt.

Email

dr.khalid64@yahoo.com

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First Name

Nancy

Last Name

Magdy

MiddleName

-

Affiliation

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt.

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City

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Orcid

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First Name

Ghada

Last Name

Mohamed

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-

Affiliation

Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt.

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Volume

1

Article Issue

4

Related Issue

691

Issue Date

2017-10-01

Receive Date

2017-07-12

Publish Date

2017-10-01

Page Start

209

Page End

2,015

Print ISSN

2357-0547

Online ISSN

2357-0539

Article File

APRH_Volume 1_Issue 4_Pages 209-2015.pdf

PDF . 641.2kB

Link

https://aprh.journals.ekb.eg/article_4042.html

Detail API

https://aprh.journals.ekb.eg/service?article_code=4042

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Publication Type

Journal

Publication Title

Journal of Advanced Pharmacy Research

Publication Link

https://aprh.journals.ekb.eg/

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Article

Created At

22 Jan 2023