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3384

Influence of siRNA Loaded Nanoparticles on the Metastasis and Growth Rate of Cancer Cell Lines

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Last updated: 22 Jan 2023

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Abstract

Objectives: The aim of this study was to investigate the ability of siRNA-loaded nanoparticles to control the capability of cisplatin-resistant cell lines to decrease cancer metastasis and cell proliferation rate. Methods: The effect of siRNA-loaded NPs on the migratory (metastasis) and proliferation (cell growth rate) activity of cisplatin-resistant cell lines compared to untreated cells was investigated using the wound healing assay and cell growth rate assay, respectively. Results: The results showed that cells were treated with siRNA-loaded nanoparticles after 24 h from starting the scratch, succeeded to cover of 59 ± 12.9, and 30 ± 6.1% only from the wound for cell line Cis 1R and Cis 12R, respectively. On the other hand of the untreated cells that were treated with positive control nanoparticles showed complete healing for the wound within the same period of time. Results of cell proliferation of both cell lines exhibited significantly (P≤ 0.001) lower growth rate after treatment with loaded nanoparticles compared to positive control nanoparticles and untreated cells. Conclusion: The obtained results indicated that siRNA-loaded nanoparticles succeeded to not only reduce metastasis but also decrease cell proliferation rate of cisplatin-resistant cell lines.

DOI

10.21608/aprh.2017.3384

Keywords

cell growth, Migration, proliferation, Scratch assay, siRNA

Authors

First Name

Mohammed

Last Name

Teiama

MiddleName

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Affiliation

Helwan University, Faculty of Pharmacy, Department of Pharmaceutics and Industrial Pharmacy, Ain Helwan, Helwan, Cairo11795, Egypt

Email

dr_mteiama84@pharm.helwan.edu

City

-

Orcid

-

First Name

Mohamed

Last Name

Nasr

MiddleName

-

Affiliation

Helwan University, Faculty of Pharmacy, Department of Pharmaceutics and Industrial Pharmacy, Ain Helwan, Helwan, Cairo11795, Egypt

Email

-

City

-

Orcid

-

First Name

Fahima

Last Name

Hashem

MiddleName

-

Affiliation

Helwan University, Faculty of Pharmacy, Department of Pharmaceutics and Industrial Pharmacy, Ain Helwan, Helwan, Cairo11795, Egypt

Email

fahima20012000@yahoo.co.uk

City

-

Orcid

-

First Name

Mohamed

Last Name

ElSayed

MiddleName

-

Affiliation

Cellular Engineering & Nano-Therapeutics Laboratory, Department of Biomedical Engineering, College of Engineering, University of Michigan, Ann Arbor, Michigan 48109, United States

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City

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Orcid

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Volume

1

Article Issue

3

Related Issue

599

Issue Date

2017-07-01

Receive Date

2017-03-01

Publish Date

2017-07-01

Page Start

171

Page End

175

Print ISSN

2357-0547

Online ISSN

2357-0539

Article File

APRH_Volume 1_Issue 3_Pages 171-175.pdf

PDF . 609.7kB

Link

https://aprh.journals.ekb.eg/article_3384.html

Detail API

https://aprh.journals.ekb.eg/service?article_code=3384

Order

6

Type

Research Article

Type Code

318

Publication Type

Journal

Publication Title

Journal of Advanced Pharmacy Research

Publication Link

https://aprh.journals.ekb.eg/

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Article

Created At

22 Jan 2023