Objective: study the effect of hydroxychloroquine (HCQ) and Artemisia extract on liver enzymes and kidney function tests.

Methods: seventy-two male mice are used in Lab as equal four groups:

- A control group diagnosed with distilled water,

- 1st group diagnosed with hydroxychloroquine (HCQ)

- 2nd group diagnosed with hydroxychloroquine and Artemisia extract

- 3rd group was diagnosed with Artemisia extract.

The time of investigation was around 6-18 days for each group of mice orally.

Results: AST, ALT, and ALP as liver enzymes increased in 1st and 2nd groups but these enzymes were low at 3rd group. Urea level increased in 1st group and decreased in 2nd group while no change was noticed in 3rd group. Also, Creatinine levels showed no significant change in all groups in comparison with the control group. After 6,12and 18 days of oral administration, the liver enzymes increased specially in 1st and 2nd groups. Also, urea concentration increased after 6, 12 and18days in 1st group. While, Creatinine levels showed no significant change in all periods.

Conclusion: Treatment of mice body at our lab with HCQ, Artemisia extract or both led to increment of liver enzymes, while urea level in mice body was high as we treated with HCQ and with low level in both HCQ and Artemisia extract.