A series of bi nuclear transition metal complexes of Co(II), Ni(II) ,Cu(II) and Zn(II) of the general composition (ML(H2O)2Cl2) where (M=Co(II), Ni(II) and Cu(II), L=(3-(4-bromophenyl)-5-(thiophen-2-yl)-4,5-dihydro-1H-pyrazole-1-carbothioamide). The new ligand was synthesized via ring closure of ((E)-1-(4-bromophenyl)-3-(thiophen-2-yl)prop-2-en-1-one) with the excess of thiosemicarbazide. The new 2-pyrazoline was identified by MS, NMR,FT-IR spectra in addition the elemental microanalyses were carried out to elucidate the chemical structure after adjusting the thin-layer chromatography in (3:1) volume ratios of eluent solvents of ethylacetate and chloroform. The solid metal complexes of the new ligand were prepared and isolated in their solid state by direct reactions of their metal chlorides with the ethanolic solution of the ligand (L). The new metal complexes of cobalt(II),nickel(II),copper(II) and zinc(II) were fully characterized by elemental analysis (C.H.N.S), FT-IR, molar conductivity measurements in DMF solvent, electronic spectra and magnetic susceptibility via Farady's method. As well as the ionization mass spectra was assigned for some complexes to investigate the expected skeletal of the complexes. The data observed from elemental analyses, flame atomic absorption and spectroscopic techniques afforded the octahedral environment around cobalt(II),nickel(II) and copper(II) ions whereas the tetrahedral geometry was adopted for ZnLCl2.The anti-microbial activities of these derivatives have been studied by screening them against bacteria like Staphylococcus aureus and Eschericheia Coli and fungi like Aspergillusnidulece and Condida albicance by Serial dilution method.