This study focuses on combine nanoscale structure with biomolecules to create new biological and nanotechnology applications. Gelatin nanoparticles was prepared by nanoprecipitaton method with various parameters and utilized the optimum as a carrier for curcumin as anticancer drug, the obtained nanoparticles had a homogenous well-defined morphology that confirmed using transmission electronic microscopy and FT-IR, as well a curcumin was loaded on gelatin nanoparticles by emulsification linkage technique in the presence of SLES as emulsifier, and the EE of the drug was controlled by the polymer concentration, the emulsifier concentration, and the drug to polymer ratio. The results showed that GNps exhibit high Encapsulation Efficiency (EE) which reached to 82%. Additionally, studying the curcumin release profile at different pH values (1.2 and 7.4) at 37 °C for 72 h showed that the amount of drug released at acidic pH 1.2 is higher than that of pH 7.4 and also the release rate had slow and sustained.