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273725

Using molecular tools to compare various concentration protocols prior to detecting SARS-CoV-2 RNA in wastewater

Article

Last updated: 01 Jan 2025

Subjects

-

Tags

Environmental chemistry

Abstract

Background: Reports showed presence of SARS-CoV-2 genetic material in wastewater. Wastewater concentration methods are optimized for detection of non-enveloped viruses so need to be adopted for enveloped viruses and their genetic material. Methods: Conventional (cRT-PCR) and quantitative real time RT-PCR (qRT-PCR) were used as readouts to compare 4 water concentration methods namely, A) filtration on negatively charged membrane followed by extracting RNA from it, B) adsorbtion-elution method, C) flocculation with skimmed milk and D) polyethylene glycol precipitation, to detect SARS-CoV-2 RNA and 229E human coronavirus (229E-HCoV) as a model for spike-containing enveloped virus from fresh and wastewater. Results: On using cRT-PCR: recovery rate of SARS-CoV-2 RNA was better using method A then B for fresh water and method B then D for wastewater. 229E-HCoV recovery from fresh water was better using method C then A and methods B then D for wastewater. On using qRT-PCR, both methods A and B were better for SARS-CoV-2 RNA recovery from both fresh and wastewater. For the 229E-HCoV methods A was the most efficient for fresh water and method B for wastewater. Conclusion: Method B is recommended for SARS-CoV-2 RNA or whole 229E-HCoV recovery from wastewater.

DOI

10.21608/ejchem.2022.165190.7030

Keywords

SARS-CoV-2, Human corona virus 229E, molecular tools, Wastewater, Concentration methods

Authors

First Name

Dina

Last Name

Abd-Elshafy

MiddleName

Nadeem

Affiliation

Water pollution research department, institute of environmental research and climate change, National research center,giza,Egypt

Email

dnanadeem@yahoo.com

City

-

Orcid

0000-0002-2777-2487

First Name

Rola

Last Name

Nadeem

MiddleName

-

Affiliation

Therapeutic chemistry department, Institute of pharmaceutical and drug industry research, National research center, Giza, Egypt

Email

rola.nadeem@hotmail.com

City

-

Orcid

0000-0000-4093-2270

First Name

Mahmoud

Last Name

Bahgat

MiddleName

-

Affiliation

Therapeutic Chemistry Department, National Research Centre (NRC), 33 EL Bohouth St. (former El Tahrir St.),

Email

mbahgatriad@yahoo.com

City

-

Orcid

0000-0002-6485-8722

Volume

65

Article Issue

13

Related Issue

37459

Issue Date

2022-12-01

Receive Date

2022-09-25

Publish Date

2022-12-11

Print ISSN

0449-2285

Online ISSN

2357-0245

Link

https://ejchem.journals.ekb.eg/article_273725.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=273725

Order

273,725

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

Using molecular tools to compare various concentration protocols prior to detecting SARS-CoV-2 RNA in wastewater

Details

Type

Article

Created At

22 Jan 2023