The green synthesis of silver nanoparticles (AgNPs) regarded as the most significant field of nanotechnology science because of the cost-effective and environmentally friendly. Methodology used in the process of the fundamentals of green nanotechnology is the use of reductive by products in the food and fruit processing industry as a cheap and dependable source of green reducing agents due to their favorable physicochemical properties and applications potential. Our study's objective was to use pomegranate to biosynthesize silver nanoparticles (PAgNPs) from (Punica granatum) Peel(waste)Extract(PPE)to explore its characterization using scanning electron microscopy and an ultraviolet-visible Spectrophotometer which revealed the development of spherical and sparsely agglomerated PAgNPs as assessed in the200–600 nm range. Antimicrobial activities where they demonstrated positive activity to varied degrees against the majority of the tested human pathogenic bacteria and fungi. The toxicity test was conducted using a Microtox analyzer 500 in aqueous solutions, and it was found that the EC50 value of Ag+ after 15 minutes of exposure was lower than the EC50 value of Ag0NPs. This showed that Ag+ was more toxic to Luminescent bacteria than PAg0NPs.