Background: Strong evidence confirmed that chronic exposure to pesticide causes adverse effects on human health such as mutagenic, cancer and even death. Glyphosate, an active ingredient in widely used organophosphate pesticide “Roundup Star", is an amino acid analog of glycine that inhibits the tryptophan metabolism in weeds. Several studies indicated that intraperitoneal administration of low dose (50 mg/kg) and high dose (300 mg/kg) of Roundup® result in significantly increased of chromosomal aberrations and micronuclei in mice. Objective: We aimed to investigate the biochemical and histopathological changes resulting from exposure to GBHs in albino mice. Methods: The study was conducted on 80 mice divided into four groups; GPC: 20 untreated mice as control group, GP50: 20 mice were treated with 50 mg/kg/bw GBHs for 3 months; GP125: 20 mice were treated with 125 mg/kg bw GBHs for 3 months and Gp250: 20 mice were treated with 250 mg/kg bw GBHs for 3 months. Blood samples were collected for assessment of the liver, spleen, kidney, brain, heart, lung and gastrointestinal tract (GIT) function including assessment the enzyme activity of Alanine transaminase (ALT), Aspartate transaminase (AST), Creatine kinase (CK-MB), alpha amylase, Lactate dehydrogenase (LDH) and Alkaline phosphatase (ALP), determination of the mean levels of creatinine, Urea, Uric acid, total protein, albumin, hemoglobin and bilirubin as well as histopathological examination of liver, kidney, spleen, brain, heart, lung and gastrointestinal tract. Results and discussion: We found that GBHs caused deleterious effects on all examined organs accompanied with significant elevation of ALT, AST, ALP, LDH, ALP, CK-MB and alpha-amylase activities as well as elevation of the mean levels of Urea, creatinine, and bilirubin levels while significant decrease of total proteins, albumin, and hemoglobin in dose dependent manner compared to control group. We concluded that GBHs causes multi-organs disorders in male albino mice.