The recent work has focused on the production of biobutanol from Colocasia esculenta peels waste using a pure strain of Clostridium acetobutylicum ATCC 824 at different concentrations of 20, 40, and 60 g/L of peels as a rich source for carbon for the fermentation process. The maximum carbohydrate content (48.16 g/L) was attained at 60 g/L of the peels. The higher biomass growth of the investigated strain (10.5 g/L) was achieved when 60 g/L of the peels were used at 72h. By increasing the peels concentrations from 20 to 60 g/L, where the resulted biobutanol had elevated from 2.11 to 12.56 g/L respectively at 72h. The process of fermentation parameters was optimized where the best pH and fermentation temperature values were 6.5 and 35oC respectively in the presence of 60 g/L of peels at 72h.