This study aims to investigate thermodynamic parameters for nano VO (II) sulfate alone and with amino acid (H2Prol) to form complexes in the solutions, and metal complexe have been characterized on the basis of elemential analysis, electronic spectra, IR spectra and various physico chemical study. The measurements of vandyl sulphate conductance have happened in binary mixed solvent with ethanol in different mass fraction 0.0, 0.2 and 0.4 (W/W) (EtOH-H2O) at four different temperatures from 298.15 to 313.15K (with a step of 5 K). The experimental data obtained by using the Fuoss-Shedlovsky extrapolation technique. All thermodynamic parameters for association were calculated, also we studied the effect of hydrogen bond formation in solution. Furthermore, biological activity measurements are executed, which benefits in determining the factors impacting the thermodynamic parameters