The environmental constrains issued recently in many countries reinforce the textile chemists and colorists to adopt eco-friendlier methods during dyeing and finishing of textile substrates. Herein, a genuine colorant extracted from mulberry leaves was used in dyeing of natural silk fabric using conventional heating as well as ultrasonic- or microwave-assisted dyeing.Adopting pre-, meta-, and post-mordanting methods, dyeing of natural silk was carried out also in presence of iron II sulphate heptahydrate as a mordant. The colour strength, colorimetric data, fastness properties, antimicrobial properties, and the resistance to ultraviolet radiation of the dyed samples were monitored. The results proved that mulberry leaves' extract is a suitable eco-friendlier colorant for dyeing of natural silk fabrics with a high colour strength and adequate fastness properties against light, washing, rubbing, and perspiration, in presence of FeSO4.7H2O as a mordant. The dyed fabrics exhibited excellent resistance to microbial attack, including Gram +ve, Grame –ve bacteria and a pathogenic fungus, as well as ultraviolet radiation.