This study investigated the potential of Fusarium oxysporum f. sp. pisi (FOP) on the removal of both important dyes, Congo red (CR) and Basic fuchsin (BF), by batch adsorption experiments. The effects of pH, contact time, and initial dye concentration were investigated. Fourier transform infrared spectroscopy (FT-IR) analysis revealed the involvement of different functional groups, mainly carboxyl, phosphate, amino and amide groups during the biosorption process. Scanning Electron Microscopy (SEM) analysis shows that the fungal mycelia have rough and porous surface. The initial pH of the dye solution strongly affected the chemistry of both the dye molecules and fungal biomass in an aqueous solution. The maximum biosorption capacities of Congo red and Basic fuchsin were found at pH 6 and 303 K, and the values were 08.72 mg/g and 32.08 mg/g, respectively. The results indicated that the bioremoval efficiency of 30 mg/L CR reached approximately 34.70% after 60 min of the exposure time; however, the maximum biosorption of 40 mg/L BF was determined to be about 79%, respectively. The contact time necessary to reach equilibrium was 60 min. Freundlich and Dubinin-Radushkevich isotherm equations well described biosorption data, and the pseudo-second order model provided the best fit to the kinetics rate. This study showed that FOP is an efficacious, eco-friendly, bio renewable and affordable biomaterial for dye removal from industrial effluents.