A green chemistry methodological approach was used in the preparation of nanostructures, which is a result of incredible and innovative medical applications. Polysaccharides were utilized as a reducing and stabilizing agent in the synthesis of gold nanoparticles (AuNPs). We used carboxyethyl chitosan (CECS) as a reducing agent and a gold nanoparticle as a capping agent in this study. CECS is made by reacting chitosan with acrylic acid in an alkaline media, as described previously. At 100 oC for 1 hour, AuNPs were made with varied concentrations of carboxyethyl chitosan (0.2 percent w/v, 0.5 percent w/v, and 1 percent w/v). The nitrogen content, carboxyl content, and FTIR spectra were used to characterize CMCS. UV spectrophotometry and TEM images were used to identify AuNPs. Finally, the cytotoxicity of the produced AuNPs was assessed using an MMT assay for cell viability and IC50 values in comparison to AuNPs synthesized via chemical techniques. The results reveal that AuNPs have a normal distribution with 15-25 nm particle size and that their cytotoxicity is reduced when made using this green approach, indicating that GNPs can be used safely in skin contact medical therapy