Anise (Pimpinella anisum L.), is a traditional famed plant belonging to Apiaceae family with eminent therapeutic potentials throughout the world. In this study, the effects of an elicitor such as yeast extract (YE), and a precursor such as phenylalanine on the growth and accumulation of bioactive compounds in Anise shoot tip cultures were identified. Shoot tip callus growing well on Murashige and Skoog (MS) medium supplemented with 1mg/l indole acetic acid (IAA) and 1mg/l 2,4-dichlorophenoxy acetic acid (2.4-D) as auxins in addition to 2mg/l of kinetin (kin). Fresh weight and increase values were progressively increased and reached the maximum (26.9 g and 52.8; 13.7 g and 26.5 respectively) at 5.46 mM/l yeast and 2mM phenylalanine concentrations compared to control treatment (12.9 g, and 24.7). Total volatile constituents content were enhanced to 43.51% at 6 mM/l phenylalanine and 45.45% at 1.82 mM/l yeast correspondingly, compared to control treatment (16.83%). Gas chromatography-mass spectrometry (GC-MS) analysis of calli cultures of Pimpinella anisum showed the presence of 20 different compound which account for the 99.9% of the total amount. 1,2-Diphenyl-5-(t-butyl) acephenanthrylene was scrutinized in all treatments. The results obtained may support the use of acephenanthrylene in traditional medicine for the treatment of various diseases and in