The present work was designed to study bifunctional reactive dyes by printing and cold pad batch method also evaluated their antibacterial activity. The main object of this research paper is to highlight synthesized dyes having excellent fixation abilities for the dyeing and printing industries to further invest upon. All dyes were reported earlier, In the synthesis of reactive dyes phenyl urea derivatives introduced in triazine ring, the dyes have excellent solubility. Synthesized dyes Z1-10 were applied on cotton fabric with cold pad batch dyeing and printing method. The fixation value of dyes in the CPB method and percentage of absorbance in the printing method of dyes were evaluated. Ten synthesized reactive dyes had a 64 % absorbance value in the print method due to the solubility behavior of dyes. The colorimetric data of dyed and printed fabric were examined in the CIELab system. The Fastness properties of dyed and printed fabrics were evaluated through standard test method. Antibacterial activity of synthesized dyes was investigated against different kinds of bacteria on dyed fabric. The synthesized dyes had good Antibacterial affinity compared with selected antibiotics as a reference standard.