Jojoba oil has an acaricidal effect on Tetranychus urticae adult female mortality, as well as antifeedant, larvicidal, and physiological effects on other insects. The goal of this study was to formulate jojoba oil to compare its effectiveness with Baraka oil, and citric acid formulations against the Two-spotted Spider Mite T. urticae. A new local jojoba oil formulation was developed as an emulsifiable concentrate (EC). Three formulations for (jojoba oil, Baraka oil and citric acid) were put to the test on eggs of T. urticae. Jojoba oil and Baraka oil were used as emulsifiable concentrates (ECs) while citric acid was used as soluble powder (SP).Jojoba oil EC showed the highest result followed by Baraka oil EC, and citric acid SP. The EC50 values were 376 ppm for jojoba oil and 1305 ppm for Baraka oil (ECs), respectively. Under greenhouse conditions, jojoba oil EC was evaluated on egg hatching and individuals of T. urticae at 1000 and 2000 ppm. After 14 days of treatment, egg hatching was totally suppressed, whereas individual inhibition was 84.4 and 91.4 % for both concentrations, respectively. As a result, the two-spotted spider mite can be combated with the new jojoba oil EC formulation.