Titanium substituted lanthanum strontium manganite LSMTO (La0.5Sr0.5Mn1-xTixO3, x= 0, 0.1) nanopowders were prepared via organic acid precursor using glycine-nitrate as fuel. The structural, microstructure, magnetic properties, anti-microbial activity as well as anti-cancer activity of La0.5Sr0.5Mn0.9Ti0.1O3 at different calcination temperatures from 800 to 1200oC for 2h were scrutinized. The ideal orthorhombic perovskite LSMTO with a space group Pbnm was proved at different synthesis conditions. However, substitution of Ti4+ ion instead of Mn4+ ion in LSMO annealed at different temperatures led to an increment of the crystallite size from 13.57 to 16.10 nm. The FT-IR spectrum elucidated that the bands around 600 cm-1 associated with Mn-O turn out broad imputed to Ti-O stretching modes. The morphology of the produced samples appeared as spherical groupings. The temperature dependence of magnetization confirms that Ti+4 ions were weakness the ferromagnetic (FM)-paramagnetic (PM) double exchange transition as the result of the break in the FM Mn3+–O–Mn4+ interactions. Molar magnetic susceptibility was notified to increase with the temperature. Subsequently, Curie temperature values were found to increase from 373 to 383 K with increasing the temperature, respectively. Eventually, LSMTO powders have cytotoxic effect on HepG-2 with IC50 value (105, 135 and 152 μg/ml) for LSMTO annealed at 800, 1000 and 1200 °C, respectively.