Zinc oxide nanoparticles were produced using green synthesis method by Aspegillus niger, F1a. The nanoparticles were purified and their characteristics were analyzed using different techniques viz. UV–visible spectrophotometry, FTIR and TEM. The morphology, structure and the dimensions of zinc nanoparticles were recorded as spherical shaped particles ranging in size from 6.35-10.5nm. Glutamines enzyme was produced biologically by the same strain used for nanoparticles production. The enzyme was purified up to 1.33 fold and 7.31% recovery. Mixture of gltaminase and zinc nanoparticles (protein corona PC) was prepared and tested on A-549 cells (Lung carcinoma). Cytotoxicity measurements have shown increasing percentage of cancer cells inhibition from 73.17 % and 88.04% by zinc nanoparticles and glutaminase enzyme, respectively independently up to 97.06% by mixture of both zinc nanoparticles and glutaminase was approved. The interaction of this protein corona was studied using EDX, NMR and TEM.