Beta
ManageSearchChatDiscover
260074

Production and characterization of L-Asparaginase enzyme from symbiont bacteria of red algae Eucheuma spinosum

Article

Last updated: 01 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

-

Abstract

Red algae are one of the marine creatures which can produce a variety of both primary and secondary compounds. It is widely known that the existence of exploitation will cause species extinction if there is no cultivation. Due to this problem, the use of bacterial symbionts of algae is one of the solutions. This study aimed to obtain bacteria isolates that can produce the L – Asparaginase (L-ASNase) enzyme. The research design was explorative using experimental laboratory methods by using solid substrate fermentation techniques. The sample used was red algae Eucheuma spinosum located on the island of Lae-Lae of Spermonde island of South Sulawesi. Analysis of the data displayed in the form of tables, graphs/images. Based on the results, two species of bacteria-derived L-Asparaginae enzyme. Protein content with the highest of the bacteria Enterobacter genus obtained from fractions 20-40% was equal to 1.780 mg/mL. The highest enzyme activity was in fractions of 20-40% with 32.011 IU/mL. The optimum temperature and pH of each was at 37 °C and pH 7.

DOI

10.21608/ejchem.2022.81705.4045

Keywords

Red algae, Bacteria, L–Asparaginase, Enzyme, Eucheuma spinosum

Authors

First Name

Mukriani

Last Name

Mukriani

MiddleName

-

Affiliation

Graduate School of Medical Science, Medicine Faculty, Hasanuddin University, Perintis Kemerdekaan Street Km. 10 Tamalanrea, Makassar 90245, Indonesia

Email

mukriani17@gmail.com

City

-

Orcid

-

First Name

Ahyar

Last Name

Ahmad

MiddleName

-

Affiliation

Chemistry Department, Mathematics and Natural Sciences, Hasanuddin University, Makassar, Indonesia

Email

ahyarahmad@gmail.com

City

Makassar

Orcid

0000-0002-0913-4721

First Name

Nur

Last Name

Asmi

MiddleName

-

Affiliation

Chemistry Department, Mathematics and Natural Sciences, Hasanuddin University, Makassar, Indonesia

Email

nurasmi0193@gmail.com

City

Makassar, Indonesia

Orcid

0000-0001-6366-1832

First Name

Harningsih

Last Name

Karim

MiddleName

-

Affiliation

Pharmacy College YAMASI, Makassar 90222, Indonesia

Email

harningsihkarim@gmail.com

City

Makassar

Orcid

-

First Name

Muhammad

Last Name

Massi

MiddleName

Nasrum

Affiliation

Department of Microbiology, Faculty of Medicine, Hasanuddin University, Makassar 90245, Indonesia

Email

nasrumm2000@yahoo.com

City

Makassar

Orcid

0000-0002-3347-6529

First Name

Prihantono

Last Name

Prihantono

MiddleName

-

Affiliation

Department of Oncology, Medicine Faculty, Hasanuddin University, Perintis Kemerdekaan Street Km. 10 Tamalanrea, Makassar 90245, Indonesia

Email

prihantono.md@gmail.com

City

Makassar

Orcid

0000-0002-8247-0457

Volume

65

Article Issue

11

Related Issue

35761

Issue Date

2022-11-01

Receive Date

2021-06-22

Publish Date

2022-11-01

Page Start

475

Page End

481

Print ISSN

0449-2285

Online ISSN

2357-0245

Article File

EJCHEM_Volume 65_Issue 11_Pages 475-481.pdf

PDF . 616.7kB

Link

https://ejchem.journals.ekb.eg/article_260074.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=260074

Order

43

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

Production and characterization of L-Asparaginase enzyme from symbiont bacteria of red algae Eucheuma spinosum

Details

Type

Article

Created At

22 Jan 2023