Three biomarkers namely; cathelicidin, neutrophil gelatinase associated lipocalin (NGAL), and Interleukin-22 (IL-22), are used as prognostic indicators for urinary tract infection (UTI). This study involved sixty patients diagnosed with UTI who attended to (AL-Sader medical city and private laboratories) in AL-Najaf province, during the period from November 2021 and January 2022. Patients were divided according to their age, gender (male and female), and control group (apparently healthy). Thirty samples exhibited negative urine cultures, all of which matched the patients' ages. The results revealed a significant increase in cathelicidin, NGAL-2 and IL-22 levels in urinary tract infection patients when compared to the control group, as well as other age groups (30-39), (40-49), and (50-59). Female patients had a significant rise (p< 0.05) in all three biomarkers when compared to male patients.