Purslane (Portulaca oleracea L.) is a plant that is distributed widely around the world. It contains many secondary metabolites that are used in treatment of many diseases. The present study was conducted to investigate the effect of plant growth regulators on calli induction, and the subsequent experiments were conducted to investigate the influence of biotic (Fusarium oxysporum) and abiotic elicitation (Mannitol) on active constituents of P. oleracea. The results showed that the best calli induced from the stem explant of P. oleracea on Murashige and Skoog's medium with benzyl adenine 1 mg / L and 2,4-dichlorophenoxyacetic acid 0.25 mg / L. On the other hand, Alkaloids became 280 mg / g in a treated calli with 1 % filtrate of fungal and (100 mil Mol) mannitol after being 140 mg / g in dry powder of calli. Flavonoids became 340 mg / g in a treated calli with 1 % filtrate of fungal with 150 mM mannitol after being 180 mg / g in dry powder of calli. Tannins became 180 mg / g in a treated calli with 1.5% filtrate of fungal only after being 180 mg / g in dry powder of calli. Elicitation studies have a promising effect in increasing yields of the secondary metabolites in P. oleracea.