Glycyrrhiza glabra roots contain high nutritive value and substantial medicinal properties such as antibacterial, antioxidant, antimalarial, antispasmodic, anti-inflammatory and anti-hyper glycemic activities. Plant samples were collected from Babylon governorate, Iraq. Confirmed classification of G. glabra and preparation of root extract were implemented in the laboratories of college pharmacy/university of Babylon. Various extracts and chemical compounds were obtained and the antimicrobial effects of G. glabra was assessed against Staphylococcus aureus, Escherishia coli, Pseudomonas aeruginosa, and Candida albicans using agar well diffusion method and concentration range of 500-3000mg/mL. Results of the chemical analysis of G. glabra root extracts proved that the aqueous extract contained saponin, flavonoids, glycosides, but lacked alkaloids and phenols. The ethanolic extract contained tannins and terpenoids. All tested isolates were sensitive to both extracts at concentration of 3000 mg/ml. S. aureus and E. coli showed higher sensitivity, maximum effective inhibition for G. glabra aqueous extract was found against S.aureus, whereby the minimum inhibition was recorded against P. aeruginosa. Regarding G. glabra alcoholic extracts, the highest effect was noticed against P. aeruginosa, while the lowest effect was noticed on C.albicans.