In this research, Indium oxide (In2O3), nickel oxide (NiO) and NiO/In2O3 heterostructure were synthesized and characterized using X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), energy dispersive X-ray spectrometry (EDS)/Map, Brunauer, Emmett, Teller (BET)/Barrett, Joyner, Halenda (BJH) and UV–vis diffuse reflectance spectroscopy (DRS) techniques. The photocatalytic degradation of Apramycin (APR) from aqueous solutions studied by photocatalysts synthesized. In order to investigate the effect of parameters in degradation of APR, were used from response surface methodology (RSM). The effect of NiO/In2O3 (0.5:1) catalyst mass (g), time (min) and pH on degradation of APR evaluated. Design expert software is the best point to achieve the maximum degradation efficiency of APR 99.99%, in optimum conditions at pH 5.01, time 27.54 (min), mass of catalyst 0.03 (g).