Applying vitamins like pyridoxine to decrease the impact of various stressors on the majority of plants has an important efficiency. Thus, this research tries to study the physiological impact of pyridoxine and mycorrhizae exogenous treatments on improving white lupine tolerance to salinity stress. Mycorrhizae was added to soil (0.0 and 7 g/pot).White lupine seedlings were foliar applied by various levels of pyridoxine (0.0, 100 and 200 mg/l) and watered by two levels of salinity (0.0 or 5000 mg/l). Irrigation of white lupine plants with saltwater resulted in significant reductions in morphological aspects, photosynthetic pigments and productivity in comparison to irrigation with tap water, meanwhile, gradually increase osmolytes, comparing to control (plants irrigated by tap water). Adding mycorrhizae to soil with the recommended dose boosted white lupine growth, certain physiological and productivity of plants irrigated with saltwater. Furthermore, exogenous pyridoxine treatment with 100 & 200 mg/l enhanced growth and seeds productivity of white lupine plants under normal irrigation and improved salinity tolerance by increasing white lupine growth and productivity via inducing photosynthetic pigments, osmolytes levels comparing to their corresponding controls. In conclusion, 200 mg/l pyridoxine showed superiority on inducing beneficial role in improving lupine plant salinity tolerance.