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215828

Possible correlation between probiotic activity of bacterial honey isolates and severe acute respiratory syndrome coronavirus 2 replication in vitro

Article

Last updated: 01 Jan 2025

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Subjects

-

Tags

Biochemistry

Abstract

Various strategies, like those using vaccines and antibiotics, have been examined for the prevention and treatment of virus's diseases, but until this moment infection control is not at sufficient level. Exopolysaccharides, especially from probiotics, became one of the most innovative approaches for antiviral agents. This research tried to highlight the effect of a probiotic polysaccharide such as levan in COVID-19 prevention. Accordingly, 5 levans types previously obtained from bacterial honey isolates were tested against COVID-19. The most promising result was recorded with levans from Pseudomonas aeruginosa {levAE] and Bacillus subtilis9A {lev9A]. The lowest cytotoxicity was obtained from lev9A {CC50=5.567e+006 mg/ml] and the most promising IC50 was obtained by levAE {10.75 mg/ml] followed by lev13M {142.5 mg/ml] then lev9A {1299 mg/ml]. The dialysis process of levAE/D greatly affected the virus inhibition activity {IC50=7.773e+006 mg/ml]. Pseudomonas aeruginosa HI1 and Bacillus subtilis 9A were highly tolerant to the acidic {pHs 2, 3] and alkaline sides {pHs 9,11]. They incubated with 0.3 bile salt for 24h and their surviving rates recorded 94% and 100% respectively. H2O2 tolerance showed that Pseudomonas aeruginosa HI1 kept 77% of its surviving while Bacillus subtilis9A remained complete surviving. The blood hemolysis and the antibiotics sensitivity tests confirmed the isolate's safety. The isolates adhere to the lung cells, this hypothesis that the isolates and their levans could share effectively in covid-19 prevention.

DOI

10.21608/ejchem.2022.115169.5225

Keywords

Levan, probiotic, COVID 19

Authors

First Name

Asmaa

Last Name

Ezzat

MiddleName

-

Affiliation

Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Dokki, Cairo, Egypt

Email

a.e-3@hotmail.com

City

-

Orcid

0000-0002-1493-0386

First Name

Noura O.

Last Name

Abdelsamad

MiddleName

-

Affiliation

Biotehnology Program, Faculty of Agriculture, Cairo University

Email

nouraabdelsamad97@gmail.com

City

-

Orcid

-

First Name

Amira A.

Last Name

Gamal

MiddleName

-

Affiliation

Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Dokki, Cairo, Egypt

Email

drmero_156@hotmail.com

City

-

Orcid

0000-0003-0315-2029

First Name

Mahmoud

Last Name

Shehata

MiddleName

-

Affiliation

Center of Scientific Excellence for Influenza Viruses, National Research Centre, 12622 Dokki, Giza, Egypt.

Email

shehata_mmm@hotmail.com

City

-

Orcid

-

First Name

Sara H.

Last Name

Mahmoud

MiddleName

-

Affiliation

Center of Scientific Excellence for Influenza Viruses, National Research Centre, 12622 Dokki, Giza, Egypt

Email

sarahussein9@yahoo.com

City

-

Orcid

-

First Name

Ahmed

Last Name

Mostafa

MiddleName

-

Affiliation

Center of Scientific Excellence for Influenza Viruses, National Research Centre, 12622 Dokki, Giza, Egypt

Email

ahmed_nrc2000@hotmail.com

City

-

Orcid

-

First Name

Mohamed A.

Last Name

Ali

MiddleName

-

Affiliation

Center of Scientific Excellence for Influenza Viruses, National Research Centre, 12622 Dokki, Giza, Egypt

Email

mohamedahmedali2004@yahoo.com

City

-

Orcid

0000-0002-5615-3212

First Name

Mona A.

Last Name

Esawy

MiddleName

-

Affiliation

Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Dokki, Cairo, Egypt

Email

mona_esawy@hotmail.com

City

-

Orcid

-

Volume

65

Article Issue

9

Related Issue

31876

Issue Date

2022-09-01

Receive Date

2022-01-08

Publish Date

2022-09-01

Page Start

467

Page End

476

Print ISSN

0449-2285

Online ISSN

2357-0245

Article File

EJCHEM_Volume 65_Issue 9_Pages 467-476.pdf

PDF . 888.3kB

Link

https://ejchem.journals.ekb.eg/article_215828.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=215828

Order

45

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

Possible correlation between probiotic activity of bacterial honey isolates and severe acute respiratory syndrome coronavirus 2 replication in vitro

Details

Type

Article

Created At

22 Jan 2023