The pure hydroxyapatite (HAp) and antibacterial co-doped hydroxyapatite [(Cu, Mg) HAp] nano-powders were synthesized by Wet chemical precipitation method. Calcium hydroxide (CaOH2) and phosphate acid (H3PO4) were utilized as starting materials, magnesium oxide (MgO) and copper oxide (CuO) represent the doping elements . The ratio (Ca/P) kept at 1.67 and PH value maintained to10 at room temperature. Pure HAp [Ca10(PO4)6OH2] and three stoichiometric compositions of the co-doped HAp were prepared which were [(Mg0.25,Cu0.75)Ca9(PO4)6OH2],[(Mg0.5,Cu0.5)Ca9(PO4)6OH2] and [(Mg0.75,Cu0.25)Ca9(PO4)6OH2]. All the prepared powders were calcinated at 800 °C for 2hrs . The structure, antibacterial activity, and toxicity with cell viability have been investigated using several characterization techniques . XRD results showed that the fabricated undoped- HAp and co-doped HAp has one (hexagonal) phase , and the FT-IR results shows functional group that confirm the results of XRD. The TEM images indicated that incorporation of Cu2+ and Mg2+ has altered the size and morphology of the resulting HAp crystals which appears elongated with dissimilar sizes. Antibacterial test indicated that co-doped HAp [(Mg0.25,Cu0.75)Ca9(PO4)6OH2] showed high activity versus E.coli and S.aureus bacteria. The MTT assay for the [(Mg0.75,Cu0.25)Ca9(PO4)6OH2] has shown superior positive role on cell viability as appears in the fluorescent image, and has acceptable antibacterial activity.