Glasses of nominal composition (6Na2O.12K2O.5MgO.20CaO.4P2O5.(53-x) B2O3. xCuO Wt%) (0 ≤ x ≤ 0.6 wt%) were successfully synthesized via the traditional melt quenching route. The synthesized samples proved to be amorphous without any tendency for crystallization through X-ray diffraction measurements. Structural changes within the glassy matrix and their ability for bone bonding were investigated through employing deconvolution analysis technique (DAT) combined with Fourier transform Infrared (FT-IR) spectral measurement to calculate the percent of tetrahedral boron change versus doping copper ion concentration. It was found that copper additions control the transformation of triangular and tetrahedral coordinated boron that increase with increasing copper content up to 0.4 wt%. UV/Vis. electronic spectral data was used to calculate physical parameters including optical energy gap, refractive index, dielectric permittivity, and other physical parameters. Antimicrobial activity against different pathogenic grams using a cup and disc diffusion method was also correlated to the copper oxide doping level. All previous investigations were used as an optimizing factor for the synthesis of bioactive borate glass suitable for wound healing and wound dressing applications.