An important direction toward change refers to replacing the synthetic components, especially the synthetic dyes, currently utilized in the textile industry that is hazardous both to humans and nature. The goal of this research was to see how effective aqueous extraction of natural color from Beetroot waste was. FTIR, UV-VIS and mass spectroscopy analysis assured the presence of the betalian and phenolic groups. Subsequently, an effort was made to develop an environmentally friendly approach for extracting betalains and phytochemicals from beetroot pomace by taking into account several experimental factors such as beetroot weight, pH, temperature, and time. The extract was utilized as a vital bio-dye to dye cotton, wool, and silk fabrics. A pseudo-second-order kinetic model (R2 > 0.99) best describes the adsorption kinetics of textiles. . The dyed fabric's color strength (K/S) values were measured; the value has reached from 9.7 to 15.6 for treated silk fiber. All colored samples were also subjected to color fastness tests, which included washing, perspiration, and exposure to light. In addition to there importance as colorants, betalains play indispensable role in human health because of their pharmacological activities as antioxidants and anti-carcinogens.