A new family of sulfur-containing plasticizers derivedfrom oleic acid and thiopropionic acid has been developed. The synthetic is related to thiol–ene addition of thiopropionic acid to the double bond of oleic acid, followedby oxidation of the sulfide group to sulfone groups. The prepared compounds [methyl 10(3-methoxy-3-oxobutanesulfonyl) octadecanoate (M3) and [ethyl 10(3-ethoxy-3-oxobutanesulfonyl) octadecanoate (M4) were characterized by Fourier Transfer Infra-Red Spectroscopy FT-IRand1HNMR. F1 and F2 are formulations of PVC with M3 and M4, respectively, in addition to F0 as blank with dioctyl phthalate (DOP) (40% wt).The mechanical properties and thermal analysis differential scanning calorimetry (DSC) of the prepared formulations films were investigated. Also in this study we evaluated the antimicrobial activity (antibacterial) and antitoxic effectof the prepared compounds. It was found that the synthesized plasticizers M3 and M4 have plasticization effect similar to DOP on PVC. The prepared M3 and M4 were found to enhance activity on bacteria growth compared to DOP. M4 was found to be safe material since it's very active against VERO line cell with IC50 value206.18 while DOP with IC50 value 197.06.