Owing to fresh water paucity, farmers are using wastewater for irrigation. This study was performed to assess whether application of Spirulina platensis, Chlorella vulgaris, leaves powder of salix and Ethylenediamine tetraacetic acid could ameliorate the harmful effects of wastewater on Jute mallow. In addition to estimating heavy metals accumulation in Jute mallow leaves (edible part). The results revealed that the contents of total soluble protein in shoot and root throughout the two growth stages, yield parameters and yield components were significantly reduced in wastewater-irrigated plant. The contents of malondialdehyde, free proline, total phenols, superoxide dismutase, catalase, peroxidase and polyphenoloxidase significantly increased in wastewater-irrigated plant than control. Wastewater-irrigated plant has higher levels of Ni, Cd, Pb and Co in leaves, which were beyond recommended limits set by international regulations and the higher rates of plant enrichment was Pb. In contrast, our treatments attenuated the adverse effects of wastewater on metabolic contents, malondialdehyde, non-enzymatic and enzymatic antioxidants, yield parameters and yield components of Jute mallow. Moreover, the concentration of Ni, Cd, Pb and Co in leaves were significantly decreased. Finally, these findings suggested that Spirulina platensis and Chlorella vulgaris treatments could be used for mitigating the harmful effects of wastewater on plants.