To determine the effectiveness of brown algae extract (Sargassum polycystum) to reduce the lipoteichoic acid (LTA) level of Enterococcus faecalis bacteria through reducing Toll-Like Receptor-2 (TLR-2) expression in fibroblast cells. This study was an in vitro laboratory experiment with a post-test-only control group study design. The initial stage of this study was the production of brown alga (Sargassum polycystum) extract with concentrations of 0.5, 0.10, and 0.15 g/ml. Each concentration of extract brown alga was given 10 g/ml of LTA and inserted into cultured fibroblast cells, then the cultured cells were incubated in a carbon dioxide (CO2) incubator for 24 hours. The amount of TLR-2 was then determined using the immunocytochemical method. The data were analyzed statistically using the Mann-Whitney test. The Mann-Whitney test showed significantly different results between treatments, with the 0.15 g/ml concentration group having an average TLR-2 value in the fibroblast cell culture of 34.00  1.73% significantly lower than the 0.05 g/ml concentration group (mean 62.33  8.08%) and 0.10 g/ml concentration group (mean 62.00  15.59). These results show that the treatment with a concentration of 0.15 g/ml was effective in reducing the LTA level of E. faecalis bacteria through TLR-2 expression in fibroblast cell culture. The brown alga (Sargassum polycystum) extract used at a concentration of 0.15 µg/ml was effective in reducing the LTA level in E. faecalis bacteria through the expression of TLR-2 in fibroblast cells.