Phyllanthus emblica is used in traditional medicine due to its bioactive constituents. This study aimed to evaluate the antimicrobial activity of the Phyllanthus emblica methanol extract and its chemical profile. The plant was extracted with 85% methanol and the total phenolic and flavonoid contents as well as antimicrobial activity on some Gram- positive, Gram-negative bacteria and some fungi species were evaluated. Also, GC-MS analysis of plant extract was administered. Phyllanthus emblica methanolic extract showed high total phenolic content (216.41± 2.1 mg GAE/g of dry extract) and total flavonoid content (32.31± 1.2 mg RE/g of dry extract). The antimicrobial activity of extract against some pathogenic microbes proved that the plant extract is effective against all species except Escherichia coli. The highest activity was showed against Aspergillus fumigatus & Syncephalastrum racemosum (fungi) as well as Staphylococcus aureus, Syncephalastrum racemosum & Streptococcus pneumonia (bacteria). GC-MS analysis of Phyllanthus emblica exhibited the presence of thirty-one bioactive compound. The major compounds are 1, 3-Dimethylindole (24.11%), Isopropyl palmitate (23.94%), Gamolenic acid (7.73%) 1, 5-Hexanediol (7.58%), 8, 11-Octadecadienoic acid methyl ester (6.66%), 2-Methylhexadecanoic acid (5.62%) and Linolenic acid (3.49%). Thus, Phyllanthus emblica may be used as a potential oral antibiotic against some pathogenic microbes.