Bioglass with the composition of SiO2-B2O3-24.5CaO-24.5Na2O–6P2O5 by varying B2O3 content on SiO2 content using the conventional melt-quenching technique. Bioglass was analyzed using X-ray diffraction, Ultraviolet (UV-Vis) spectroscopy, and Fourier transform infrared (FTIR). Tested samples were subjected to two-step-controlled crystallization route to obtain their respective glass-ceramic derivatives. The crystalline nature of the glass-ceramic was tested via x-ray diffraction analysis. FTIR spectral data of analysed samples before and after controlled crystallization. Optical absorption spectral data was used to estimate different physical parameters associated with the compositional variation of boron atoms at the expense of silicon atoms. Density, molar volume, molecular mobility, average boron–boron, boron atom molar volume, ion concentration, polaron radius, and field strength were also estimated and correlated to the structural variations.