Modified Hench's bioglass of the basic composition xAgO (45 B2O3, 24.5 CaO, 24.5 Na2O, 6 P2O5) as (x= 0.1, 0.2, 0.4, 0.6, and 0.8 wt.%) were successfully prepared via the melt quenching route. Immersion in phosphate solution (PS) varying times (1→4 weeks) was used to assess the in-vitro behavior of the bulk glassy samples with different silver oxide content. X-ray diffraction XRD, scanning electron microscopy (SEM), and other spectroscopic measurements including Fourier transform infrared (FT-IR) analytical techniques and UV/visible optical measurements before and after prolonged immersion times were used to estimate the bioactive behaviors and to explain the efficiency of bone-bonding. Besides, antimicrobial tests against pathogenic grams were examined and silver ions show varying levels of action against certain bacteria. It was noticed that prolonged immersion periods cause distinct variations that link the vibrational bands as a result of a preferential attack or ion exchange and modifier replacement by silver ions in the glass network causing the hydroxyapatite formation as an indication for bone-bonding ability. Antimicrobial experiments reveal that studied material can be used efficiently as an antibacterial agent.