Pot experiments were carried out during two successive seasons of 2018/2019 and 2019/2020 at the experimental farm of Horticulture Research Institute, Agricultural Research Center, Giza, Egypt to study the effect of different types of fertilizers, traditional NPK (t-NPK) at 2.0 g/pot, nano NPK (n-NPK) at 0.5 and 1.0 g/l, active dry yeast extract (ADYE) at 5.0 and 10.0 g/l, moringa leaves extract (MLE) at 5.0 and 10.0%, and some combinations (ADYE at 5.0 g/l + MLE at 5.0%, MLE at 10% + n-NPK at 1.0 g/l and ADYE at 10.0 g/l + n-NPK at 1.0 g/l) as well as untreated plants (control) on flowering and some chemical constituents of Gladiolus grandiflorus cv. Rose Suprem. The results showed that MLE at 10% produced the highest values for number of florets/spike and spike stem length (cm) followed by MLE at 10% + n-NPK at 1 g/l which gave the highest values in case of spike stem diameter (cm), flower diameter (cm), fresh and dry weights of cut spike (g). On the other hand, chemical constituents of chlorophyll a, b, carotenoids of fresh leaves (mg/g), N, P, K and total carbohydrates (%) were influenced by different fertilization treatments, the mastery in this concern was to application of MLE at 10% + n-NPK at 1 g/l. It could be recommended that application of Gladiolus grandiflorus cv. Rose Suprem by MLE at 10% + n-NPK at 1 g/l to get the best results regarding flowering and chemical constituents