Hypercholesterolemia is a major risk factor for the development of atherosclerosis; a number of pharmacological and non-pharmacological (including dietary) approaches being employed to reduce it. Up until now, the trials to prove the hypocholesterolemia effect of probiotics have not been convincing. There are only few data suggesting that the supplementation of the diet with functional food products containing probiotic bacteria lower LDL-cholesterol (LDL-C) concentration in patients with moderately elevated cholesterol concentration in plasma. The aim of the present study was to evaluate in vitro the anti-atherosclerotic effect of a probiotic (Lactobacillus rhamnosus, casei, plantarum, and reuteri), prebiotic (Flaxseed & Cinnamon, green coffee, ginger, and green tea extracts) & combination mix using MCF-7cell line. Recording data showed that probiotic compound administration to hyperlipidemia: a significant decrease of the total serum cholesterol & triglycerides, a significant increase of the serum antioxidant potential. In conclusion, our data support the administration of probiotic Lactobacillus bacteria and prebiotic and combination to decrease serum cholesterol, triglycerides, and lipid droplets increase the antioxidant potential in hyperlipidemia subjects.