Recently, interest has increased in natural metabolites compounds from plants for industrial and pharmaceutical use rather than synthetic compounds. Tanacetum sinaicum Del. Is a wild plant found in many places in Egypt, especially south Sinai, in Saint Catherine, on rocky slopes and it has yellow tubular flowers and woolly leaves. Using different chromatographic Techniques, extracted, separated, cleaned, and defined two phenolic compounds as a result of these experiments were obtained the following compounds were 4',5-dihydroxy-6,7-dimethoxyflavone, and 3,4-dihydroxy-cinnamic acid. The structures of these compounds were identified by using, UV, NMR, and mass spectroscopy. The quantitative estimation of total phenolics, total flavonoids, total alkaloids, total saponins, and total tannins showed presence in a good amount in Tanacetum Sinaicum flowers when compared to other plant parts. The phytochemical screening of crude extract and the corresponding sub-fractions to investigated alkaloids, anthraquinones, carbohydrates, cardiac glycosides, flavonoids, glycosides, reducing sugars, resin, saponin, starch, sterols, tannins, and terpenoids of different parts of Tanacetum Sinaicum showed that crude extract of all parts and chloroform fraction of flowers are rich in all active constituents when compared to other parts and fractions.