The present study was conducted to evaluate field efficacy of the recommended doses of five acaricides (cyflumetofen, 20% SC; pyridaben, 15%EC; spiromesifen, 24% SC; spirodiclofen, 24% SC and fenpyroximate, 5% EC) followed by dissipation study for cyflumetofen and pyridaben, against Panonychus citri (McGregor) on lemon trees. The mean number of motile stages was significantly (p < 0.05) reduced in all treatments after spraying the acaricides compared with the control. The cyflumetofen and spirodiclofen showed tangible efficacy decreasing P. citri movable stages numbers. During two consecutive seasons, the highest efficacy was recorded seven days after application, while the lower one recorded after three days of application. Results showed that the population of P. citri examined in the treated plot was these pesticides had been used, was significantly reduced. Based on these results, the cyfumetofen and spirodiclofen are to be recommended for citrus red mite control programs. The analytical procedures were done using QuChERS method and LC-MS/MS for determination. The recoveries ranged between 92 and 117% with ≤ 4.2 % RSD and ± 6% combined uncertainty. The half-lives were calculated using the first-order kinetic model for both pesticides, cyflumetofen (5.33 days) and pyridaben (7.29 days).