However the adverse impact of organic pollutants and their occurrence in the food chain in Egypt, scarce studies have dealt with these pollutants in water resources. The aim of this work is the non-targeted organic compounds investigation in Assiut Governorate groundwater, Egypt. Thirty-four samples were collected and analyzed for their chemical oxygen demand (COD), and three of them were selected for the non-targeted survey of organic compounds by GC-MS instrument. The COD values ranged from 0 to 216 mg O2/l, indicating the pollution of some samples with organic compounds. The GC-MS results indicated the occurrence of plastic, petroleum, pesticides and pharmaceutical residues in the analyzed samples. The source of these compounds may be household effluents, improper disposal of wastewater through absorption septic tanks, improper application of panned pesticides and leakage from fuel tanks. Finally, the groundwater needs targeted investigation of organic pollutants in the study area.