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196781

Effect of Galectin-3 Inhibition on Hepatocyte Nuclear Factors 4α and 1α Expression in Liver after Acetaminophen Long-term Induced Toxicity

Article

Last updated: 01 Jan 2025

Subjects

-

Tags

Biochemistry

Abstract

Acetaminophen (APAP) induced liver injury can occur through acute or repeated administration of high doses. Hepatocyte nuclear factors HNF4α and HNF1α are key transcription factors involved in regulating the expression of drug metabolism enzymes and are affected by proinflammatory cytokines. Meanwhile galectin-3 (Gal-3) is a β-galactoside-binding lectin implicated in the regulation of macrophage activation and secretion of inflammatory mediators. This study aimed to evaluate the impact of galectin-3 inhibition on HNF4α and HNF1α gene expression after long-term administration of APAP, and to investigate the resulted effect on APAP induced hepatotoxicity. The study was performed on thirty two Wistar rats divided equally into four groups; control group, modified citrus pectin (MCP) (Gal-3 inhibitor) receiving group, APAP receiving group, and APAP plus MCP receiving group. APAP induced inhibition of liver HNF4α and HNF1α gene expression, and elevation of liver Gal-3 and tumor necrosis factor- α levels (TNF-α), depletion of reduced glutathione and its maintaining enzymes, besides elevation in levels of serum liver function parameters and appearance of necrotic areas in the liver. Gal-3 inhibition preserved HNF4α and HNF1α gene expression, TNF-α, and each of reduced glutathione and its maintaining enzymes near normal levels. It also significantly reduced APAP induced hepatotoxicity.

DOI

10.21608/ejchem.2021.92576.4382

Keywords

HNF4α, HNF1α, galectin-3, Inflammation, Acetaminophen long-term administration

Authors

First Name

Mahmoud

Last Name

Abdel-Monem

MiddleName

A.

Affiliation

Department of Medical Biochemistry, National Research Centre, Cairo, Egypt.

Email

mahmoud199018@gmail.com

City

Cairo

Orcid

0000-0003-4540-1206

First Name

Ahmed

Last Name

Salem

MiddleName

M.

Affiliation

Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt.

Email

ahmedsalem47@hotmail.com

City

-

Orcid

0000-0002-8789-4654

First Name

Karam

Last Name

Mahdy

MiddleName

A.

Affiliation

Department of Medical Biochemistry, National Research Centre, Cairo, Egypt.

Email

ka.mahdy@nrc.sci.eg

City

-

Orcid

0000-0001-6591-2041

First Name

Gamila

Last Name

El-Saeed

MiddleName

S.M.

Affiliation

Department of Medical Biochemistry, National Research Centre, Cairo, Egypt.

Email

gs.elsaeed@nrc.sci.eg

City

-

Orcid

0000-0001-6236-2873

First Name

Abdel-Razik

Last Name

Farrag

MiddleName

H.

Affiliation

Department of Pathology, National Research Centre, Cairo, Egypt.

Email

ah.farrag@nrc.sci.eg

City

-

Orcid

0000-0002-6121-450X

First Name

Nahla

Last Name

Hassan

MiddleName

S.

Affiliation

Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt.

Email

nahla_samir@hotmail.com

City

-

Orcid

0000-0002-9530-0828

Volume

65

Article Issue

3

Related Issue

29682

Issue Date

2022-03-01

Receive Date

2021-08-25

Publish Date

2022-03-01

Page Start

375

Page End

384

Print ISSN

0449-2285

Online ISSN

2357-0245

Link

https://ejchem.journals.ekb.eg/article_196781.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=196781

Order

35

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

Effect of Galectin-3 Inhibition on Hepatocyte Nuclear Factors 4α and 1α Expression in Liver after Acetaminophen Long-term Induced Toxicity

Details

Type

Article

Created At

22 Jan 2023