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204350

Therapeutic Efficacy Of Curcuma And Pomelo Loaded Chitosan Nanoparticles In Intestinal Murine Trichinellosis

Article

Last updated: 22 Jan 2023

Subjects

-

Tags

Biochemistry

Abstract

The majority of the medications used to treat trichinellosis had little bio-availability and a elevated resistance level. This work compared the usage of Curcuma longa extract, pomelo peels extract, and their chitosan (CS) nanoparticles (NPs) versus the existing albenazole treatment for experimental trichinellosis. A total of 49 male albino mice were used in this study. Healthy controls (GI), infected untreated controls (GII), Albendazole-treated infected group (GIII), infected group receiving pomelo peels extract (IV), infected group receiving pomelo peels extract-loaded CS NPs (GV), infected group receiving Curcuma longa extract (GVI), infected group receiving Curcuma longa extract-loaded CS NPs (GV), infected group receiving Curcuma longa extract loaded CS (G VII).They were divided into seven experimental groups: healthy control (GI), infected untreated control (GII), Albendazole administered infected group (GIII), infected group receiving pomelo peels extract (IV), infected group receiving pomelo peels extract-loaded CS NPs (GV), infected group receiving Curcuma longa extract (GVI), and infected group receiving Curcuma longa extract-loaded CS NPs (GVII). The extracts were given orally to experimentally infected mice at a rate of 100 mg/kg/day commencing 24 hours after infection and continuing for 14 days on parasite burden, histological alterations in the gut, skeletal muscle, and oxidative stress. Except for the healthy control group, all groups were infected orally with T. spiralis larvae at a dose of 200 larvae per mouse. On the 35th day following infection, all groups were sacrificed. The results demonstrated a significant reduction in adult worm count during the intestinal phase in all treated groups. The group treated with pomelo NPs had the highest reduction rate of adult worms, at 94.13 %. For the group treated by pomelo NPs, it showed better rank compared to non-infected group and albendazole administered infected group, as well as reduced larval count during muscle phase, the highest reduction rate of larvae was 80.2% for the group treated by Curcuma NPs. The same concept has been counted for the encysted phase. This was reflected further by improved parasite burden, histopathological alterations, cytokines and oxidative stress compared to control group and Albendazole administered infected group. TNF-α, IL-6, NOS2, SOD3, α-GST concentrations have been investigated in sera and showed that TNF-α, IL-6, and NOS2 levels have been elevated significantly (P >0.01) in infected group (GII), while SOD3 and α-GST levels have been significantly decreased compared to negative control (GI) (P>0.01). Treatment of infected mice with Curcuma and pomelo NPs have been significantly improved (P>0.05) all parameters levels in sera and back them close to the original levels of negative control. Finally, we can conclude that introduction of Curcuma and pomelo NPs could be of therapeutic potential use against trichinellosis.

DOI

10.21608/ejchem.2021.102400.4750

Keywords

Key words: Trichinella spiralis, albendazole, Chitosan nanoparticles, Curcuma longa, pomelo peels

Authors

First Name

Wafaa

Last Name

Abd El-Hamed

MiddleName

-

Affiliation

a.Biological and Environmental Sciences Dept., Faculty of Home Economics, Al-Azhar University, Egypt

Email

memo_modern2010@yahoo.com

City

-

Orcid

-

First Name

Ahmed

Last Name

Abd-Rabou

MiddleName

-

Affiliation

cHormones Department, Medical Research Division, and Stem Cell Laboratory, Center of Excellence for Advanced Science, National Research Centre, 12622, Giza, Egypt.

Email

ahmedchemia87@yahoo.com

City

-

Orcid

0000-0002-9875-7591

First Name

Asmaa

Last Name

Faramawy

MiddleName

-

Affiliation

c Nutrition and Food ScienceDept., Faculty of Home Economics, Al-Azhar University, Egypt.

Email

allahasmaa2020@gmail.com

City

-

Orcid

-

Volume

65

Article Issue

2

Related Issue

28751

Issue Date

2022-02-01

Receive Date

2021-10-23

Publish Date

2022-02-01

Page Start

551

Page End

564

Print ISSN

0449-2285

Online ISSN

2357-0245

Link

https://ejchem.journals.ekb.eg/article_204350.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=204350

Order

56

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023