Vitiligo is the loss of functional melanocytes from epidermis. Acrofacial type occurs when depigmentation affects parts apart from the body centers as face, head, hands and feet. Vitamin D may have a role in both acrofacial vitiligo and Covid 19. The present study designed to investigate the role of serum levels of vitamin D, Calcium (Ca), phosphorous (Ph), matrix metalloproteinases 1 and 2 (MMPs 1, 2) and tissue inhibitor matrix metalloproteinase -1 (TIMP1) in acrofacial vitiligo during Covid-19. Activity of MMP1, MMP2 and TIMP1 calcium and phosphorous levels were measured in serum. 40 control subjects and 40 patients' suffering acrofacial vitiligo, whose ages ranged from 17-36 years with matching sex and skin phototype all were examined. Vitamin D and Ca levels showed significant decline in their levels in acrofacial vitiligo patients when compared to controls. Additionally, TIMP1 and MMP2 levels displayed significant decrease compared to control subjects, while elevation in MMP1 level was recognized. However, Phosphorous showed no significant difference between controls and cases. No significant alteration between patients and control in terms of sex & age was detected in the present study. We concluded that the selected measured parameters were suggested to participate in controlling and regulating the processes of melanocyte activity and migration in acrofacial vitiligo. These parameters were correlated to each other, since significant reduction in vitamin D & Ca was associated with the decrease in MMP2 and TIMP1 as well as significant increase in MMP1 in vitiligo patients. However, no significant differences were noticed in phosphorous levels in acrofacial vitiligo when compared to control subjects. Significant reduction in vitamin D was also correlated to Covid-19.