Human wellbeing is jeopardized by microbial pollution. Antimicrobial compounds produced from a number of nanoparticles (NPs) were shown to have strong antimicrobial efficacy in studies. There are possible effects of nanostructured metal oxides on microbial contamination reduction are well established. This research aimed to look at the antimicrobial properties of zeolite (nanoparticles in zeolite) against E. coli (negative bacteria) and Staphylococcus aureus (positive bacteria). Real or synthetic crystalline Aluminum silicates with ion-exchanging properties are known as zeolites. The current search focuses on zeolites' uses in biomedical applications, Normal zeolites have been used in a variety of applications. Normal zeolites have been used in the pharmaceutical industry and medicine for many years. Since several biomedical processes are linked to ion exchange, adsorption, and catalysis, natural zeolites are poised to make major advances in these fields in the near future. Natural silicate compounds, such as zeolite, have been found to have a wide range of biological activities and have been used to treat diarrhea and as a vaccine is adjuvant. The search includes the usage of microwave plasma in enhancing the result of (NPS) solution of zeolite in three concentrations. The result of the Atomic Force Microscope (AFM) shows that the diameters of the particles become smaller with solution exposed to plasma and with increasing the time expose and the energy gap decrease over time exposing so the diameter of the inhibitions zone increase with increasing the time exposing to plasma.