Cationic copolymers based on both styrene and N,N-dimethylaminoethyl methacrylate monomers were synthesized. Styrene monomer is reactedwith N,N- dimethylaminoethyl methacrylate (DMAEMA) monomer through conventional free radical polymerization. This reactionwas performedusing styrene to DMAEMA ratios of 1:0.25, 1:0.5, 1:1, and 1:2 molesto form copolymers SD0.25, SD0.5, SD1, and SD2, respectively.The resultant copolymers are characterized by Fourier transforms infrared (FTIR) spectroscopy, and proton nuclear magnetic resonance (1HNMR) spectroscopy. The surface morphology is studied using field emissionscanning electron microscope (FESEM). The positive charge was introduced to the formed copolymers through alkylation with hexyl bromide forming the corresponding cationiccopolymers; CSD0.25, CSD0.5, CSD1, and CSD2, respectively.The cationic copolymers are characterized by FTIR, 1HNMR, wide angle X-ray diffraction (WAXRD), thermogravimetric analysis (TGA) and FESEM. The antibacterial activity ofthe copoylmers and their cationic forms are tested againstEscherichia coliserotype O145 (E. ColiO145) and Staphylococcus aureus (S. aureus)strains.CSD0.5 and CSD1 are found to have promising antibacterial activity against both bacterial strains.