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193174

Application of the yeast comet assay in Testing some food additives for genotoxicity by comet assay in yeast

Article

Last updated: 01 Jan 2025

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Subjects

-

Tags

Biochemistry

Abstract

Food additives of various types are frequently used in the food sector. Their use is justified by the need to preserve, color, or sweeten a variety of foods. Despite the fact that some additives have been demonstrated to be cytotoxic, they are nevertheless utilized in practice. The effects of monosodium glutamate (MSG), sodium benzoate (SB), and saffron on several yeast haploid knockout strains were studied in this study (YKO). We used the Comet test method to find the optimum amounts at which this set of dietary additives could cause DNA damage. Three regularly used dietary additives were found to efficiently damage DNA. We also evaluated the sensitivity of higher eukaryotic cells to the genotoxic effects of these chemicals with yeast. The comet assay exhibited a better sensitivity of yeast cells, which was undeniably confirmed. The genotypes of haploid (knockout) yeast strains were chosen based on the (Clustal Omega Multiple Sequence Alignment EMBL-EBI) alignment of human and yeast gene sequence homology.

DOI

10.21608/ejchem.2021.90428.4315

Keywords

YKO, MSG, saffron, SB, Comet assay, genotoxic

Authors

First Name

shimaa

Last Name

rashad

MiddleName

elsayed

Affiliation

Genetic Engineering and Biotechnology, National Research Centre, Giza, egypt

Email

shimaar30@gmail.com

City

-

Orcid

0000-0003-1277-5922

First Name

fatthy

Last Name

abdel-tawab

MiddleName

mohamed

Affiliation

Department of Genetics, Faculty of Agriculture, Ain Shams University, Kaliobeya, Egypt.

Email

fatthy38@yahoo.com

City

Cairo

Orcid

-

First Name

eman

Last Name

fahmy

MiddleName

mahmoud

Affiliation

Department of Genetics, Faculty of Agriculture, Ain Shams University, Kaliobeya, Egypt

Email

emanfahmy27@yahoo.com

City

Cairo

Orcid

-

First Name

Ashraf

Last Name

Attallah

MiddleName

Gamil

Affiliation

Microbial Genetics Department, National Research Centre, Dokki, Giza, Egypt

Email

ashrafgamil2000@yahoo.com

City

Cairo

Orcid

-

First Name

Ekram

Last Name

Ahmed

MiddleName

Sallah Eldin

Affiliation

Cell Biology Research Dept., Genetic Engineering and Biotechnology Division, National Research Centre , Dokki , Giza

Email

ekrams@hotmail.com

City

Giza

Orcid

-

First Name

abdelhamed

Last Name

haggran

MiddleName

abdelalem

Affiliation

Department of Microbial Genetic, Genetic Engineering and Biotechnology Division, National Research Center, Giza, Egypt.

Email

ahaggran@yahoo.com

City

-

Orcid

-

Volume

64

Article Issue

12

Related Issue

27765

Issue Date

2021-12-01

Receive Date

2021-08-12

Publish Date

2021-12-01

Page Start

7,649

Page End

7,667

Print ISSN

0449-2285

Online ISSN

2357-0245

Article File

EJCHEM_Volume 64_Issue 12_Pages 7649-7667.pdf

PDF . 1.1MB

Link

https://ejchem.journals.ekb.eg/article_193174.html

Detail API

https://ejchem.journals.ekb.eg/service?article_code=193174

Order

80

Type

Original Article

Type Code

297

Publication Type

Journal

Publication Title

Egyptian Journal of Chemistry

Publication Link

https://ejchem.journals.ekb.eg/

MainTitle

Application of the yeast comet assay in Testing some food additives for genotoxicity by comet assay in yeast

Details

Type

Article

Created At

22 Jan 2023