This research focuses on preparing and studying the behavior of non-woven polyacrylonitrile (PAN) nanofibers after adding γ- Fe2O3 nanoparticles to (PAN / N, N dimethylformamide (DMF)) solution with (1.45, 4.3, and 7.14) wt. %. In order to achieve the morphology properties, textural directionality, the bonding between iron oxide particles and the PAN matrix, thermal, crystalline, and magnetic properties we did the Field-Emission Scanning Electron Microscopy (FE-SEM), Fourier Transform Near-Infrared Spectroscopy (FT-NIR), Differential Calorimeter Scanning (DSC), X-Ray Diffraction (XRD), and Vibration Sample Magnetometer (VSM). Laboratory analyses demonstrated the significant influence of iron oxide nanoparticles on the characteristics and performance of the composite nanofibers in terms of reduced nanofiber diameter from 109.38±29.70 to 78.17±36.898 nm, the disappearance of beads. In addition to an increase in the crystallinity from 82.43 to 94.28 % accompanied by a larger crystalline size as a result of the polymeric fibers acquiring high magnetic properties after strengthening them with iron oxide nanoparticles, the saturation magnetization (Ms) increases with the increase of iron oxide loading in the nanofibers from (1.426 emu/g) at 1.45 wt.% γ- Fe2O3 to (6.85 emu/g) to 7.14 wt.% γ- Fe2O3 .