Ciprofloxacin is the most potent fluoroquinolone and was seen active against a broad range of bacteria. Several researchers revealed that ciprofloxacin can cause a chondrotoxic effects and supplementation with magnesium, calcium, zinc or vitamin E can diminish the induced chondrotoxicity. The aim of the present study was to assess the rat's serum magnesium, calcium, zinc, and vitamin E, after the subcutaneous injections of ciprofloxacin to male Wistar rats. Ten rats were used in the study and divided into two equal groups. On day 32 of age, all the animals in the control group were subcutaneously injected by physiological saline, while the animals in the study group were subcutaneously injected by 600 mg/kg b.w. of ciprofloxacin eight hours apart. On day 34 of age, rats were anesthetized and blood collection by cardiac puncture was taken. The present study revealed that mean serum levels for rat's magnesium, calcium, and zinc in the control group were 2.258±0.199 mg/dl, 9.416±0.369 mg/dl, and 95.51±1.81 µg/dl respectively, and the mean serum levels for rat's magnesium, calcium, and zinc in the study group were 2.138±0.257mg/dl, 9.254±0.292 mg/dl, and 94.19±2.929µg/dl respectively; The mean serum levels for vitamin E in the control and study group were 23.772±1.029 and 21.97±1.419 ng/ml, respectively. Statistical analysis showed that ciprofloxacin hydrochloride caused a non-significant decrease in rat's serum magnesium, calcium, zinc, and vitamin E levels (p>0.05). The research concluded that ciprofloxacin chondrotoxicity was not caused by the decrease in rat's serum magnesium, calcium, zinc, and vitamin E levels, and other researchers are recommended to measure their levels in the chondroid tissue after ciprofloxacin therapy.