This study presents an environmentally benign bioactive dyeing and finishing of bio-mordanting cotton fabric via hematoxylin dye extracted from Haematoxylum Campechianum L. medicinal tree. A facile approach to fabricate bio-mordanting cotton fabrics based on gelatine and aloe vera for sustainable green dyeing with hematoxylin dye to achieve a wide range of functional properties and environmental benefits was explored. Optimization of dyeing conditions in terms of salt concentration and pH was conducted. Also, build-up properties and fastness characterization haswere studied in the presence and absence of bio-mordants. The natural functional groups of the dye extract were investigated by FTIR spectral analysis. The results reflect the success of bio-mordant chemical modification and mechanism for dyeing process in the course of increasing K/S values associated with improving colorfastness to washing and light as compared to unmordant fabric. All the dyed bio-mordanting fabrics in this work showed great potential value-adding applications in terms of excellent UV protection, antioxidant activity, and high antibacterial activity. The outcomes of this study deal to have implications to the eco-textile green dyeing for the achievement of healthy and sustainable environment applications.