Human epidermal growth factor receptor 2 (HER2) is a transmembrane tyrosine kinase growth receptor protein, Positive HER2 status has been linked with aggressive tumor behavior and resistance to cytotoxic and endocrine therapies. Breast cancer is a complex disease and the major cause of mortality in women worldwide. There are several subtypes of breast cancer and many options for treatment. This study aimed to know the hormonal changes associated with HER2+ve breast cancer in pre and postmenopausal Iraqi women. This study included 80 healthy women and 78 newly diagnosed breast cancer patients, 35 postmenopausal and 43 premenopausal. Depending on the HER2 test the patients were divided into two groups, positive and negative. The group of patients with positive (HER2) test was adopted and the negative group was excluded. Our result shows that there are a significant decrease in progesterone and a non-significant increase in Estradiol, FSH, and LH in premenopausal HER2+ve breast cancer patients but there is a significant increase in estradiol, FSH, and LH in postmenopausal HER2 +ve breast cancer patients. There is a significant decrease in TSH within the normal range in both pre and postmenopausal patients compared with a control group. there is a significant negative relationship between progesterone, and FSH, a negative relationship between progesterone and LH in premenopausal patients with HER2 positive test. Also, there is a significant negative relationship between progesterone and TSH, a negative relationship between progesterone, estradiol, and FSH. And a positive relationship between progesterone and LH in postmenopausal patients with HER2 positive test.